The most famous case I know of was the HIV protease. My grad school PI used to use it as an example in class.
Science. 1992 Jun 5;256(5062):1445-8. Total chemical synthesis of a D-enzyme: the enantiomers of HIV-1 protease show reciprocal chiral substrate specificity [corrected]. Milton RC, Milton SC, Kent SB. http://www.ncbi.nlm.nih.gov/pubmed/1604320 Kelly ******************************************************* Kelly Daughtry, Ph.D. Post-Doctoral Fellow, Raetz Lab Biochemistry Department Duke University Alex H. Sands, Jr. Building 303 Research Drive RM 250 Durham, NC 27710 P: 919-684-5178 ******************************************************* On Wed, Feb 15, 2012 at 12:41 PM, Jacob Keller < j-kell...@fsm.northwestern.edu> wrote: > Are there any all-D proteins out there, of known structure or > otherwise? If so, do enantiomer-specific catalyses become inverted? > > JPK > > On Wed, Feb 15, 2012 at 8:05 AM, David Schuller <dj...@cornell.edu> wrote: > > Wukovitz & Yeates (1995) Nature Struc. Biol. 2(12): 1062-1067 > > predicts that the most probable space group for macromolecular > > crystallization is P -1 (P 1-bar). All you have to do to try it out is > > synthesize the all-D enantiomer of your protein and get it to fold > properly. > > > > > > On 02/14/12 18:36, Prem Kaushal wrote: > > > > > > Hi > > > > We have a protein that crystallized in P21212 space group. We are looking > > for some different crystal forms. We tried few things did not work. Now > we > > are thinking to mutate surface residues. Anybody aware of any software > which > > can predict the mutations that might help in crystallizing protein in > > different space group, please inform me. > > > > Thanks in advance > > > > Prem > > > > > > -- > > > > > > > > -- > > ======================================================================= > > All Things Serve the Beam > > ======================================================================= > > David J. Schuller > > modern man in a post-modern world > > MacCHESS, Cornell University > > schul...@cornell.edu > > > > -- > ******************************************* > Jacob Pearson Keller > Northwestern University > Medical Scientist Training Program > email: j-kell...@northwestern.edu > ******************************************* >
