Dear Jürgen,
Am 07.02.12 16:58, schrieb Bosch, Juergen:
<snip>
Then one last remark, LN2 versus cryo-stream freeze. Dipping in LN2
leads to a quicker freeze of your material.
</snip>
Are you sure? There was a publication by Warkentin et al. [1] about a
cold gas layer above liquid nitrogen that reduces the expected cooling
rate a lot!
My very personal experience is, that cryo-cooling in the N2-stream
worked better for me than in LN2 in a variety of projects - but the
reason could just be me ;-)
Best regards,
Dirk.
[1] Matthew Warkentin, Viatcheslav Berejnov, Naji S Husseini, and Robert
E Thorne: "Hyperquenching for protein cryocrystallography", J. Appl.
Crystallogr., 39, 805-811 (2006)
--
*******************************************************
Dirk Kostrewa
Gene Center Munich
Department of Biochemistry
Ludwig-Maximilians-Universität München
Feodor-Lynen-Str. 25
D-81377 Munich
Germany
Phone: +49-89-2180-76845
Fax: +49-89-2180-76999
E-mail: kostr...@genzentrum.lmu.de
WWW: www.genzentrum.lmu.de
*******************************************************
