Dear Theresa, Gary Gilliland's paper on cryosalts would seem to be useful for your problem.
http://scripts.iucr.org/cgi-bin/paper?en0028 Also, I have used 15% glycerol in a synthetic mother liquor to effectively freeze crystals grown in 2M Ammonium Sulfate. Another method that Jim Pflugrath teaches is to use ordinary table sugar (sucrose) dissolved in the reservoir solution to cryoprotect your crystal. A webinar is located on the rigaku website here: http://www.msc.com/protein/webinar-001.html Also, Hampton Research has a good list of tips in the Tech Support portion of their webpage. www.hamptonresearch.com Whichever you choose, good luck with your crystals! Bryan -------------------------------------------------------------------------- Confidentiality Notice: This message is private and may contain confidential and proprietary information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorized use or disclosure of the contents of this message is not permitted and may be unlawful. -----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Theresa H. Hsu Sent: Sunday, February 05, 2012 5:49 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Freezing crystal Hi all Is there a list of conditions to be tried *first* for cryoprotectant? My crystals diffract at room temperature capillary but no in 30% PEG 400. Crystals are from 2 M ammonium sulfate. Thank you. Theresa
