Hi CCP4 list,
Thanks everyone who have answered my post concerning to mutagenesis.
From quick reading most of the answers, the following seems to be a
consensus:
1) Do not concentrate your PCR product;
2) Too much DNA and/or impurities like salts or whatever can inhibits
transformation;
3) Purify your PCR product before transformation if possible or use 3 of
4 microL of it. This is more or less the amount of DNA showed in the
uploaded image.
Kind regards,
Fred
P.S.: I'll let you know the results.
