Sorry I misunderstood that, I thought you wanted to pellet cells. Well in that case my reply would have been get some 50ml Nalgene tubes (I think they are good to 50000xg) for an SS-34 rotor and spin your lysate at the maximum g force you can get for 30 minutes to get a good pellet. This will obviously not work with Falcon tubes though. Regarding the Falcon tubes I think you are pretty much at the limit I think the BD ones are only good for 10000xg.
Jürgen On Jan 31, 2012, at 12:37 PM, Raji Edayathumangalam wrote: 9000 rpm translates to 13,000 g on this centrifuge/rotor. I am not referring to pelleting bacterial cells. My question is about centrifuging bacterial lysates and some recommendations for sturdy tubes. Thanks. Raji On Tue, Jan 31, 2012 at 12:02 PM, Bosch, Juergen <jubo...@jhsph.edu<mailto:jubo...@jhsph.edu>> wrote: To how many g does your 9000 rpm translate ? Perhaps that's the problem ? 10 minutes @ 5000xg for pelleting cells is more than enough in my opinion. Jürgen On Jan 31, 2012, at 11:59 AM, Raji Edayathumangalam wrote: Hi Folks, Are you any favorite brands out there for crack-resistant 50mL centrifugation tubes. It seems we are having recurring episodes of Falcon and Corning tubes cracking even at 9,000 rpm, which is the maximum speed possible with our rotor. I have used Falcon tubes for years in the past without problems and I want to be able to spin down bacterial lysates without a mess. Any suggestions for tubes that have worked well in your experience? Thanks, Raji -- Raji Edayathumangalam Instructor in Neurology, Harvard Medical School Research Associate, Brigham and Women's Hospital Visiting Research Scholar, Brandeis University ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742<tel:%2B1-410-614-4742> Lab: +1-410-614-4894<tel:%2B1-410-614-4894> Fax: +1-410-955-2926<tel:%2B1-410-955-2926> http://web.mac.com/bosch_lab/ -- Raji Edayathumangalam Instructor in Neurology, Harvard Medical School Research Associate, Brigham and Women's Hospital Visiting Research Scholar, Brandeis University ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://web.mac.com/bosch_lab/
