You can try zanuda from York's site. www.ysbl.york.ac.uk/YSBLPrograms/index.jsp
It may help. It may be that you have a twinned crystal. As far as I know best available test for twinning is L-test (truncate produces this test). When you go from P1 to P212121 if L-test shows increased twinning then it may mean that you are overmerging. regards and I hope it helps Garib On 19 Jan 2012, at 12:06, 조기준 wrote: > Dear all, > > I have a data, and it has R/Rfree problem. > The data could be processed as P212121 and P1. > The resolution was 2.9, and unit cell parameters were a=73.527, b=90.035, > c=237.980, α=β=μ=90 and a=73.709, b=90.099, c=238.172, α=89.939, β=89.945, > μ=89.993 for P212121 and P1, respectively. > R/Rfree was 22.3/29.4 for P1 after several refinements. > However, I couldn’t decrease R/Rfree below 30.0/37.6 for P212121. > There were almost no differences on monomer structure and crystal packing > between P212121 and P1 after MR, and the electron densities followed trace of > peptide chain well. > Can anybody suggest me about this problem? > > Thank you. > Ki Joon Cho > > Garib N Murshudov Structural Studies Division MRC Laboratory of Molecular Biology Hills Road Cambridge CB2 0QH UK Email: ga...@mrc-lmb.cam.ac.uk Web http://www.mrc-lmb.cam.ac.uk
