Le 11/01/12 12:23, Federica Basilico a écrit : > Hi everyone, > > I have crystals of a protein of 176 residues, with 5 Cys, 8 His, 3 Met. > Native crystals grow in 10% MPD, 100mM Bicine pH 9.0. They show a nice > diffraction, but appear to be perfectly twinned. > I have crystallised a SeMet derivative, but I have not been able to > collect sufficiently good data, to get the phases. > Thus, I was thinking of trying with some heavy atom soaks. > Given the characteristics of my protein and crystallisation conditions, > which compounds and conditions would you advice as worth testing? > I am a beginner in crystallography, so all your suggestions would be > precious to me... > Thanks in advance, > > Federica > > -------------- > Federica Basilico > Ph.D. student > Department of Experimental Oncology > European Institute of Oncology > Via Adamello 16 > 20139 Milan (Italy) >
Hi, This site may help: http://hatodas.harima.riken.go.jp/ -- Miguel Architecture et Fonction des Macromolécules Biologiques (UMR6098) CNRS, Universités d'Aix-Marseille I & II Case 932, 163 Avenue de Luminy, 13288 Marseille cedex 9, France Tel: +33(0) 491 82 55 93 Fax: +33(0) 491 26 67 20 mailto:miguel.ortiz-lombar...@afmb.univ-mrs.fr http://www.afmb.univ-mrs.fr/Miguel-Ortiz-Lombardia
