Hello Lisa, Could the 10-20A be a result of cryo damage as opposed to true packing disorder? (try collecting a few images at room remp)
If its cryo damage, improve your cryo / try different cryoprotectants. If its not cryo, just screen about a thousand different crystals from around the same conditions as is giving you 10-20A. There will undoubtedly be some in there that are much much better - I'd say even better than 3A. Cheers, C On Fri, Jan 6, 2012 at 9:38 AM, LISA <science...@gmail.com> wrote: > Hi all, > > I have a DNA binding protein. I get crystals of this protein by > co-crystallization with different dsDNAs. But all the crystals have very > poor resolution, about 10-20A. I tried to purify protein-DNA complex before > setting trays, but it didn't work.The shape of my crystal is not bad. > Please give me some suggestion. Thanks. > > lisa > > > > > >
