Curious, how did you assess that your crystals only have DNA? F
On Nov 21, 2011, at 8:59 AM, umar farook wrote: > Dear All, > > I have been trying to crystallize protein DNA complex, but all the time i end > up with DNA crystals. Even i changed the length of DNA many times but still > no complex, DNA only crystallizes! Does anybody has idea, why do DNA > crystallize by itself ? My protein behaves very nicely, Dynamic Light > Scattering always shows nice values implies homogenous but once i tried to > ran acidic native page but it shows little bit aggregated. The protein is > highly hydrophilic and soluble, and has only three cysteines, is it there any > possibility of aggregation due to cysteine, when overexpressed in E.coli ? > and one more thing i mixed protein and DNA together and ran agarose gel to > see any gel shift, indeed there is a binding, but when i take the same thing > to set drops, only DNA crystals. Kindly suggest me, what could be done. > > > Thanks & Regards, > Umar Farook.S --------------------------------------------- Francis E. Reyes M.Sc. 215 UCB University of Colorado at Boulder
