Thank everyone for trying to help me! I am going to synchrotron. As Bernie said, I will try to use the closest distance, and 5-10deg.
Thanks again! Pengfei On 11/9/11 5:13 PM, "Santarsiero, Bernard D." <b...@uic.edu> wrote: You can collect 5-10deg images, and go ahead and collect 360deg. I usually use 1-3 seconds exposures, depending on the diffraction. The detector is set as close as possible, to get data to 0.8-0.9A resolution. For a MAR CCD 300mm detector, that's around 90-100mm, with the wavelength set at 0.8A, or around 15.5KeV in energy. You can process it with HKL2000 or XDS easily. Bernie On 11/9/11 7:36 PM, "Xiaopeng Hu" <huxp...@mail.sysu.edu.cn> wrote: X-ray machine designed for protein usually is not good for small molecule. Just go find a chemistry department/school, they can do it for you in one day. On 11/9/11 5:15 PM, "Kris Tesh" <kris.t...@att.net> wrote: Pengfei, The first thing to consider is what system you have and is it capable of collecting data to high enough resolution. So, what data collection system is available? Kris Kris F. Tesh, Ph. D. Department of Biology and Biochemistry University of Houston On Wed, November 9, 2011 3:28 pm, Pengfei Fang wrote: > Dear All, > > > I have a small molecule single crystal. I want to solve its structure by > x-ray diffraction. > > > Could you please teach me how to collect the diffraction data? > > > I have some experience with protein crystals. But it's the first time for > small molecule. > I don't how to set the parameters, like oscillating angle. > And are there any key points I should pay special attention to? > > > Thanks in advance! > Pengfei -- Bernard D. Santarsiero Research Professor Center for Pharmaceutical Biotechnology and the Department of Medicinal Chemistry and Pharmacognosy Center for Structural Biology Center for Clinical and Translational Science University of Illinois at Chicago MC870 3070MBRB 900 South Ashland Avenue Chicago, IL 60607-7173 USA (312) 413-0339 (office) (312) 413-9303 (FAX) http://www.uic.edu/labs/bds
