Dear Stephen, Unfortunately you will find that the MOSFLM tab is not very helpful. The "Flip" part of the operation generates images that are not readable by MOSFLM - I think it may have been needed for older versions of the Bruker image format.
Fortunately, it is perfectly possible to generate images suitable for MOSFLM using FrmUtility.exe although it is a two-step process. - Optionally, copy the images you wish to convert into a new folder. I like to do this since I like to keep an unaltered copy of my raw data and this is easier than recovering from the backup images made during unwarping. - Now you will need to unwarp the images. Select the Unwarp frame tab and a suitable project folder and image range. You will need a spatial correction file (this will be a p4p file if you are using Proteum software). It must be in the same directory as you images for FrmUtility.exe to find it. Normally it will be created in a sub-directory called "work" so you will need to copy it. If you cannot find this, any p4p file generated by the same detector should work. - Finally You need to convert the image format to one compatible with MOSFLM. Select the Convert format tab, and use your unwarped images as input. Select the options "Bruker/BIS format" and "2-byte (MOSFLM)" and hit convert. Hope that helps, Ed. -- Dr. E.D. Lowe Department of Biochemistry University of Oxford South Parks Road Oxford, UK OX1 3QU e:edward.l...@bioch.ox.ac.uk t: +44 (0) 1865 613288 f: +44 (0) 1865 613201 On 31/10/2011 04:56, "Dr. STEPHEN SIN-YIN, CHUI" <chui...@hkucc.hku.hk> wrote: >Dear all, > >I know somebody had asked such question before, can anyone of you can >remind me >the way of converting Bruker in-house data (*.sfrm) to the format >readable by >MOSFLM in CCP4i by using the GUI version of FrmUltility.exe? > >When I input the sfrm, and choose the tab "MSOFLM", i did not change any >setting, and it asked for P4P or SPIN, then I choose "LINEAR". Also for >the tab > >_IX, I also select "LINEAR". Finally I pressed "Unwarp, Flip & Convert" >button >to convert it, the following message come: > >FATAL dimensions of correction frame(s)different than data frame >Spatial table is 0x0, while data frame is 1024x1024 > >Please kindly advise, many thanks > >stephen > > >-- >Dr. Stephen Sin-Yin Chui (徐先賢) >Assistant Professor, >Department of Chemistry, >The University of Hong Kong, Pokfulam Road, >Hong Kong SAR, China. >Tel: 22415814 (Office), 22415818 (X-ray Diffraction Laboratory)
