Len, May be you have already done this. I would closely check my crystallization conditions and also check the pH of the cryo. In some cases, during cryoprotection the pH of the original drop may drastically different than the cryo solution. Also, sometime back, we were exploring different cryoprotectant conditions for sensitive crystal and came across this - http://www.xtals.org/crystal_cryo.pdf by Artem.
HTH Harkewal On Wed, 26 Oct 2011 11:46:08 -0500, Leonard Thomas <[email protected]> wrote: > Hi All, > > I have run into a very sensitive crystals system when it comes to > cryo protecting them. I have run through the usual suspects and > trays are going to be setup with a cryo protectant as part of > crystallization cocktail. The one problem that seems to be > occurring is that the crystals crack as soon as they are transfered > out of the original drop. I am running out of ideas and really would > love some new ones. > > Thanks in advance. > > Len > > Leonard Thomas Ph.D. > Macromolecular Crystallography Laboratory Manager > University of Oklahoma > Department of Chemistry and Biochemistry > Stephenson Life Sciences Research Center > 101 Stephenson Parkway > Norman, OK 73019-5251 > > [email protected] > http://barlywine.chem.ou.edu > Office: (405)325-1126 > Lab: (405)325-7571
