I could not agree less. There is constant development of the software for refinement that allow to do things that were not possible or were not necessary in the past such as intelligent refinement of occupancies of mutually exclusive sites, entities and conformations. I frequently remeasure lysozyme crystals. I use them as a test system for the beam lines, new detectors, novel software developments, refinement improvement etc. Sometimes I am collecting data in quite different wavelength than of existing structures. And what about diffraction data from a chemically modified lysozyme molecule? They are good data that show evolution of the beam line stations if they are keeper in historical order. To store them all, or not to store at all… Storage of the diffraction data is not a drinking club with muscle-bound selectors outside :-) Felix Frolow Dr Felix Frolow Professor of Structural Biology and Biotechnology Department of Molecular Microbiology and Biotechnology Tel Aviv University 69978, Israel
Acta Crystallographica F, co-editor e-mail: mbfro...@post.tau.ac.il Tel: ++972-3640-8723 Fax: ++972-3640-9407 Cellular: 0547 459 608 On Oct 18, 2011, at 12:52 , Chris Morris wrote: > Some crystals are hard to make, so storing all the data the best way to get > reproducibility. On the other hand, no one needs more images of lysozyme. So > using the same standard for every deposition doesn't sound right. > > The discussion should be held on the basis of overall cost to the research > budget - not on the assumption that some costs can be externalised. It is too > easy to say "you should store the images, in case I want to reprocess them > sometime". IT isn't free, nor is it always cheaper than the associated > experimental work. The key comparison is: > > Cost of growing new crystals + cost of beam line time > > With: > > Cost of storing images * probability of processing them again > > At present, detectors are improving more quickly than processing software. > Sample preparation methods are also improving. These forces both press > downward the probability that a particular image will ever be reprocessed. > > regards, > Chris > ____________________________________________ > Chris Morris > chris.mor...@stfc.ac.uk > Tel: +44 (0)1925 603689 Fax: +44 (0)1925 603634 > Mobile: 07921-717915 > Skype: chrishgmorris > http://pims.structuralbiology.eu/ > http://www.citeulike.org/blog/chrishmorris > Daresbury Lab, Daresbury, Warrington, UK, WA4 4AD
