Hi everyone:
I was hoping I could get your opinion on the performance of the small
approx. 3ml gel filtration columns from GE Healthcare. We currently have
an Akta fplc and we would like to do small runs using small volumes of
sample. As far as I know we have two options:
1. use the Superdex 15/150 columns
these appear to have a slightly lower resolution (the no. of theoretical
plates is about 25000 m-1)
these apparently can be directly hooked up to our Akta fplc
2. use Superdex PC 3.2
these have somewhat higher resolution (the no of theoretical plates is
30000 m-1)
these can only be hooked up using a special "Precision Holder" equipped
with titanium end fittings (which I have heard clog easily?)
What is your experience with these columns?
Which option of the two do you recommend (cost is not a huge issue, but
resolution and overall performance is)?
Have you seen significant band broadening when using these small columns
with a regular fplc (rather than Akta's microFPLC)?
I would greatly appreciate your comments! Many thanks...
Bests,
Alex
.
