Hi Peter, Both BeF3(-) and AlF4(-) need to be made fresh, or used from frozen. It should not be stored at room temperature. While using, you may wish to keep it on ice. If you are using Vanadate, that should be fresh (Methods in Enzymology).
Also kindly note that unlike AlF4(-), which is a single species; BeF3(-) is a mixture of BeF3.(H2O)-, BeF2(OH)- etc. It can make a difference in kinetics of binding (usually a two step binding is observed). Look at old ATPase and GTPase papers such as: Characterization of the aluminum and beryllium fluoride species which activate transducin. Analysis of the binding and dissociation kinetics.<http://www.ncbi.nlm.nih.gov/pubmed/1551879> Antonny B, Chabre M. J Biol Chem. 1992 Apr 5;267(10):6710-8. PMID: 1551879 Cheers, Partha On Tue, Oct 4, 2011 at 5:05 AM, Peter Hsu <hsuu...@u.washington.edu> wrote: > Sorry for the very off topic and dumb question, but does anyone know if > BeCl2 needs to be prepared fresh for use (making BeF3) or can it be stored > as a solution stock at room temperature/frozen? > > Thanks, > Peter >
