Hi Jacob, Once I had an issue with introuding CO2 into crystals and I was able to get it bound by putting a small piece of dry ice into the well solution and keeping the drop under CO2 pressure using nextal trays with screw cap. Please note in this case CO2 didn't get bound by using pressure cell. This could imply that CO2 gets incorporated into these crystals during crystal growth.
regards, Mathews ________________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob Keller [j-kell...@fsm.northwestern.edu] Sent: Sunday, October 02, 2011 1:56 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Bicarb at low pH Dear Crystallographers, I would like to soak my crystals in bicarbonate (a possible substrate), but the crystals have grown--and only grow--in pH 5.2-6.0, so the bicarb/CO2 will just keep evolving out of the solution and reliquishing its hydroxyls until the pH is elevated sufficiently out of range. Does anyone have a clever way of getting bicarb into these crystals? Grow them under CO2? Transfer them to higher pH, and hope for the best? Jacob Keller
