Hi Florian,

Have a look at Serial Cloner - it's free, runs on OS X, Linux and Windows, and 
is really quite powerful - including the ability to export single or multiple 
sequences to FASTA format text files (however, it can only align two sequences 
at the time I'm afraid):

        http://serialbasics.free.fr/Serial_Cloner.html

HTH, Luca

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Luca Jovine, Ph.D.
Assistant Professor & EMBO Young Investigator
Karolinska Institutet
Department of Biosciences and Nutrition & Center for Biosciences
Hälsovägen 7, SE-141 83 Huddinge, Sweden
Voice: +46.(0)8.524-81136  FAX: +46.(0)8.6081-501
E-mail: [email protected]
W3: http://jovinelab.org
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On 27 Sep 2011, at 17:42 , Florian Schmitzberger wrote:

> Dear All,
> 
> What type of software are people commonly using these days for vector/plasmid 
> map editing, making/visualizing vector maps, and aligning (small to medium 
> size) DNA sequencing data? Preferably, it should not be too expensive and be 
> able to write text files, readable by other programs.
> 
> I am familiar with VectorNTI, which is great for vector visualization and 
> editing; but I find it somewhat expensive. Sequencher seems good to quickly 
> align DNA sequences (such as from DNA sequencing) with templates, but is not 
> free. I have been using ApE for while for alignments, but aligning many 
> sequences is more cumbersome than in Sequencher; I have not tested if 
> Sequencher is good at visualizing and editing plasmid maps.
> 
> Ideally, I would like to have a single program for both purposes (vector 
> editing and DNA sequence comparison). Does something like that exist? What 
> are the alternatives to above programs?
> 
> Thank you in advance.
> 
> Florian
> 
> -----------------------------------------------------------
> Florian Schmitzberger, PhD
> Biological Chemistry and Molecular Pharmacology
> Harvard Medical School
> 250 Longwood Avenue, Seeley G. Mudd 123 
> Boston, MA 02115, US
> Tel: 001 617 432 5603
> 
> 
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> 
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> 

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