agree, any crystallisation idea is worth pursuing, given you have or can make enough sample to try it with. having said that, wouldn't you tend to select for the same crystals as the seed, i.e. crystals of the component on its own? have you tried limited proteolysis of your sample, incl. a bit of protease in the drop - or can you think of ways to stabilise the complex?Mark
Quoting Ed Pozharski: > On Wed, 2011-09-21 at 18:04 +0100, Peter Hsu wrote: >> Or is this just a crazy/bad idea? > > If there is one thing that I learned about crystallization, is that very > few ideas are so crazy that they are bad (i.e. not worth trying). Well, > if dried seaweed and ground horse hair are good for seeding, I don't see > how actual protein crystal seeds can be dismissed. > > > > -- > Oh, suddenly throwing a giraffe into a volcano to make water is crazy? > Julian, King of Lemurs > Mark J van Raaij Laboratorio M-4 Dpto de Estructura de Macromoléculas Centro Nacional de Biotecnología - CSIC c/Darwin 3, Campus Cantoblanco 28049 Madrid tel. 91 585 4616 email: mjvanra...@cnb.csic.es
