-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Obayed,
it is not clear to me whether with 'nothing is working' you mean to concentrate the solution even further or whether you mean that you do not get crystals from 7mg/ml. If you refer to the former, I would proceed with crystallisation trials at 7mg/ml. Tim On 09/21/2011 05:57 AM, Obayed Ullah wrote: > Hi all > > I am working with a human protein (not membrane protein) which is forming gel > at higher concentration. I am not able to concentrate the protein even more > than 7 mg/ml. It become like jelly which is hard to pipette out. > > I have gone through all the suggestions which you guys proposed very recently > for another member. what i mean that i already have tried different > temperature, pH, salt concentration, reducing agents (DTT, TCEF, in different > concentration), stability analysis. But unfortunately nothing is working. > > One think i want to try to add some detergent to it. If you guys have any > experience about it then please suggest the name and/or kind of detergent for > the protein. Any other suggestion also appreciated which might help. > > cheers > > > M. Obayed Ullah > E.mail: obayed.o...@yahoo.com > obayed.o...@gmail.com > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.11 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFOeaBKUxlJ7aRr7hoRAngZAKD42xoMTzC80OvrOQUwfQ48g4cuRgCg2t5X VVOf9Obqj5eMK5QEwsWbMtQ= =sjqK -----END PGP SIGNATURE-----
