-------- Original Message -------- Subject: Structure problem Date: Tue, 13 Sep 2011 08:55:47 +0000 From: #HEW KAI LI KELLY# <klh...@e.ntu.edu.sg>Hi, I am facing some problems in solving my structure now, so I am wondering if anyone is able to give me any tips and tricks on this matter. My protein-DNA complex structure diffracted to 1.5A. There are 4 missing residues, 2 on each terminal. There is no twinning in the data. The angles, the bonds, the rotamers and the Ramachandran plot are okay too. I am using molecular replacement for the phasing and the sequence homology between my protein and my homologous model is 33%. The electron density map for the protein looks very nice and there is also nice density for the DNA. Rfree converged from the initial 39%. However, Rfree refused to go down any further and it's still around 30-31%. Does anyone have any suggestions for me? Thank you in advance! Warmest Regards, Kelly Hew
Hi,pls check out <http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Refinement>
To get more specific help, you'll have to tell us much more - number of residues and bases, spacegroup, ... (at least) everything that would end up in "Table 1" of your paper describing the structure, and in the header of the PDB file.
HTH, Kay -- Kay Diederichs http://strucbio.biologie.uni-konstanz.de email: kay.diederi...@uni-konstanz.de Tel +49 7531 88 4049 Fax 3183 Fachbereich Biologie, Universität Konstanz, Box M647, D-78457 Konstanz This e-mail is digitally signed. If your e-mail client does not have the necessary capabilities, just ignore the attached signature "smime.p7s".
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