Hi, David, What is the common cause of knackered SEC column? Will equilibrizing a buffer containing 150 mM NaCl directly into a 20% EtOH or vise versa cause the problem. There was no problem just after packing the column.
Nian On Sun, Aug 28, 2011 at 9:24 AM, David Briggs <drdavidcbri...@gmail.com>wrote: > Following on from Roger's fine suggestions: > > 8. Your column is knackered. Can you see fine lines or cracks in the > column? Good packing is v.important for SEC columns. > > HTH, > > Dave > > > ============================ > David C. Briggs PhD > Father, Structural Biologist and Sceptic > ============================ > University of Manchester E-mail: > david.c.bri...@manchester.ac.uk > ============================ > http://manchester.academia.edu/DavidBriggs (v.sensible) > http://xtaldave.wordpress.com/ (sensible) > http://xtaldave.posterous.com/ (less sensible) > Twitter: @xtaldave > Skype: DocDCB > ============================ > > > > On 28 August 2011 10:25, Allan Pang <a.p...@qmul.ac.uk> wrote: > > Hi there everyone, > > > > What does it mean when you have proteins eluting in almost the whole > column > > volume of S200? > > > > I ran a gel with fractions from 8ml to 20ml and saw band for my protein > all > > throughout. > > > > Judging peaks on chromatogram is not useful as it doesn't have any > aromatic > > rings. > > > > Cheers, > > > > Allan > > > > -- > > Allan Pang > > > > PhD Student > > > > G35 Joseph Priestley Building > > Queen Mary University of London > > London > > E1 4NS > > > > Phone number: 02078828480 > > >
