Dear All
I was working on a Human protein and expression and
solubility is good in E.coli and purification is One step (His-Tag), and i
need to cleave the Histag before screens, if not
the protein will precipitated and Aggregated, but after trying for 1.2 years
i have crystals and they are with Organic solvents, (10 conditions), these
crystals are inter grown like *broccoli *shaped and i tried seeding, but
it is not successful, and even i tried with additive screen but the result
is the same .... is there is any idea to increase the size and shape of
my protein crystals.
Any suggestions will be helpful for me
Thanks in Advance
