Dear Eswar, You could try to slow down crystallization somehow. There are several ways to accomplish this:
1) increase drop size 2) add small amounts of glycerol (1-5% v/v) 3) cover the reservoir solution with paraffin or silicon oil or a mixture of both in order to reduce the vapor-diffusion rate 4) find another additive that would change the crystallization regime (e.g. kosmotropic/chaotropic agents) Good luck! christian On 08/18/2011 04:24 AM, eswar reddy wrote: > Dear All > > > I was working two domain protein and i have an > hexagonal look like crystals from additive screen, now i am facing > problem these crystal are growing in 2 minutes even in cold room even > at lower protein concentration @2mg/ml . and i have an smeary data of 7 > Å, and we thinking one domain might be flexible and other is stable > > is there anyway to increase the crystal packing .... > > Any suggestions are welcome. > > Eswar Reddy > -- __________________________________________________________________ Dr. Christian Biertümpfel Research Fellow Vaccine Research Center/NIAID/NIH phone: +1 301 594 8726 40 Convent Dr, Rm. 4613B fax: +1 301 480 2658 Bethesda, MD 20892-3027 USA __________________________________________________________________
