Hi Opher,
I seem to recall some old papers in which glutarldehyde was used (in the
RESERVOIR only) in order to promote crystallization of proteins with suspected
inter-domain flexibility. Unfortunately, I don't have specific references in
mind.
Cheers,
Boaz
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84105
Israel
E-mail: bshaa...@bgu.ac.il
Phone: 972-8-647-2220 Skype: boaz.shaanan
Fax: 972-8-647-2992 or 972-8-646-1710
________________________________________
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Opher Gileadi
[opher.gile...@sgc.ox.ac.uk]
Sent: Monday, August 15, 2011 5:56 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Protein crosslinking before crystallization
Hi all
Does anyone have experience or insights on intramolecular crosslinking of
proteins before crystallization? The idea is to lock a felxible (multidomain)
protein into a restricted conformation.
Thanks,
Opher
