Hi Ivan Did you use microseeding with *random *solutions?
If not see the following paper by Obmolova and Co about exactly this, microseeding with Fab complexes, http://journals.iucr.org/d/issues/2010/08/00/bw5361/bw5361.pdf For more subtle variations in using microseeding with complexes see http://pubs.acs.org/doi/abs/10.1021/cg2001442 Good luck Patrick On Thu, Jul 28, 2011 at 4:41 AM, xaravich ivan <xaravich.i...@gmail.com> wrote: > Hi everyone, > I have been trying to crystallize Fab:antigen complex( 50kda:90kDa) complex > and initially got needle clusters which after microseeding gave me single > crystals but they are very small and I could not repeat the results. I have > been using HEPES buffer at pH 6.8 to do the final SEC purification step of > the complex before setting trays. > I was wondering whether there are some other buffers (that one could suggest > eg tris-hcl etc) which have given decent positive results when crystallizing > Fab complexes.Though I have gone through individual papers (case by case) to > get some idea, It would be great if anyone could direct me to a > comprehensive literature towards studying the crystatllization conditions of > Fab complexes. > Equally, people who have considerable experience could suggest a list of > must do steps for such problems which have routinely been practiced in their > lab > > Also what is a good storage condition for the excess complex that you want > to use later? > I would really appreciate any suggestion,help, direction. > Thanks > ivan -- patr...@douglas.co.uk Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36
