Thank you very much everyone for the replies. Ok. Here are more details on the work:
1) Crystallization was done using counter diffusion method- in a capillary (for both Space and Ground experiments) 2) Vapor diffusion method was done only for Ground experiments, and produced both Shape 1 and Shape 2 3) The ground crystals usually grow in two forms- as nice single crystal (Shape 1) and another one as needle-like long rods (Shape 2). 4) In space, only Shape 1 crystal was formed 5) Both Ground and Space experiments- temperature was fixed at 20 deg. Space experiment- temperature was reported to us once a week. 6) Shape 2 crystals (the rods) diffracted much better, with much lower mosaicity range, produced good data processing as compared to Shape 1 (Shape 1 Space crystal's mosaicity range= 0.5-0.96! ) 7) Both Shape 1 and Shape 2 have different cell dimensions, with different volumes (Shape 1= ~ 2X of Shape 2) 8) Both structures from Shape 1 and Shape 2- solved by molecular replacement methods- no major structural changes between them Thank you. Regards, Fairolniza p/s: And thank you, Dr. Snell for the paper. I already have the paper and it has been one of my favorite references :) On Sun, Jul 10, 2011 at 10:58 PM, Artem Evdokimov <artem.evdoki...@gmail.com > wrote: > I would not rule out pure chance. Crystals of the same protein can and > often do grow in two (or more!) different forms, from 'the same conditions' > and often in the very same drop. In this case it's the same space group, but > presumably different cell dimensions? Until a significant number of crystals > from each condition (lab vs space) are analyzed, there's no reason not to > believe that it was just luck :) > > Arten > > On Sun, Jul 10, 2011 at 9:53 AM, Jim Pflugrath > <jim.pflugr...@rigaku.com>wrote: > >> ** >> OK, same space group, but you didn't indicate what the unit cells were. >> They are different, right? >> >> ------------------------------ >> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of >> *ferrol shariff >> >> *Sent:* Sunday, July 10, 2011 3:10 AM >> *To:* CCP4BB@JISCMAIL.AC.UK >> *Subject:* [ccp4bb] Same protein, different molecule numbers per ASU >> >> Hello and good day to everyone! :) >> >> I have some general questions on crystallography work. I hope you don't >> mind giving me some ideas. >> >> I have solved my lipase protein both ground-grown crystals and space-grown >> crystals with good resolutions (1.4A and 2.2A). They are the same protein >> from the same source, same purification methods, and produced crystals from >> the same crystallization conditions (except the gravity part). >> >> From the data, it shows that both of them belong to the same space group >> P212121. But they have different number of molecule per asymmetric unit. >> Ground crystal= 1 molecule/ASU, Space crystal= 2 molecules/ASU. At the >> moment i have problem explaining this issue. Is it normal to have such >> results? Same protein with different number of molecule/ASU? >> >> I've been trying to get some references on this matter but so far i don't >> really get anything that can directly explain it. Furthermore, do i need to >> relate this with the gravity effect? >> >> I hope you don't mind sharing some experiences on crystallography >> especially regarding this matter. >> >> Thank you very much >> >> -- >> FAIROLNIZA >> >> "The advantage of the emotions is that they lead us astray, and the >> advantage of science is that it is not emotional" >> -Oscar Wilde, The Picture of Dorian Gray, 1891 >> > > -- FAIROLNIZA "The advantage of the emotions is that they lead us astray, and the advantage of science is that it is not emotional" -Oscar Wilde, The Picture of Dorian Gray, 1891
