I also got something shown in pic.3 and supposed to be some oil droplet,when I 
tried to break them I found that was actually a hemisphere type material 
attached to the coverslip like a 2D circle and sphere like shape in the hanging 
drop, a small hanging-drop within a large one.Ammonium sulfate was used as 
precipitant,even I put it to run SDS-PAGE and it was (may be a quasi-crystal 
of) the protein of interest.

Reply via email to