Post-doctoral position in Structural biology of RNA decay components.
Location: Orsay, near Paris (45 minutes by regular trains), France
A 2,5 years post-doctoral position, funded by the ANR (Agence
Nationale pour la Recherche), is available to study structural aspects
of protein-protein and protein-RNA complexes involved in eukaryotic
mRNA decay.
For this purpose, the successful candidate will combine structural
biology methods (X-ray crystallography and small angle X-ray
scattering) together with biophysical approaches (ITC, DSC, MALLS,
fluorescence polarization, CD, …). All these technologies are
currently available at the state-of the-art structural biology and
biophysical platforms implemented in the host institute IBBMC
(Institute for Biophysics and Molecular and Cellular Biochemistry,
CNRS & University Paris-Sud), i.e. 3 nanodrop crystallization robots
(Tecan, Cartesian and Mosquito), a Formulatrix crystal imager, an
ITC200, an in-house SAXS apparatus, … . The lab is also well equipped
for protein production in E. coli or eukaryotic systems (P. pastoris
and baculovirus) and protein purification (several AKTA purification
systems).
The project will be carried-out under the supervision of Dr Marc
Graille at IBBMC (Orsay, Paris-Sud University, France) in close
collaboration with Dr Dominique Durand (IBBMC) for SAXS studies and Dr
Séraphin (IGBMC, Illkirch, France) for functional studies. For recent
publications related to the subject by Dr Graille and co-workers, see
van den Elzen et al; Nat. Struct. Mol. Biol, 2010; Henri J et al, J.
Biol. Chem., 2010; Rispal D et al, RNA, 2011 & Liger D, et al, Nuc.
Acids Res., 2011.
The IBBMC, an institute from CNRS and University Paris-Sud (one of the
largest and most renowned French universities), is located in a green
and timbered campus, 25km south from Paris. The lab is within 10
minutes walk from the train station, with regular connections to Paris
city centre (45 minutes). The IBBMC is 2km away from the recently
built French synchrotron SOLEIL, where we have regular access to
protein crystallography (Proxima-1) and SAXS (Swing) beamlines.
The successful candidate will have a PhD in protein biochemistry,
biophysics, or structural biology. He/she should express strong
interest in working at the interface between physics and biology.
Expertise in RNA production and purification will be very much
appreciated.
Net annual salary: around 27,000€.
Starting from January 2012.
Duration: 30 months.
Applications (including CV, covering and reference letters) or
information requests should be sent to Marc Graille (marc.grai...@u-psud.fr
) and Dominique Durand (dominique.dur...@u-psud.fr) before 15th of
September, 2011.
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Dr Marc Graille, CR1-CNRS,
Equipe "Fonction et Architecture des Assemblages Macromoléculaires"
IBBMC, CNRS UMR8619, Bât. 430 Université Paris-Sud 91405 ORSAY
Tel: 33 (0)1 69 15 31 57 / Fax: 33 (0)1 69 85 37 15
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
