Hi Obayed,
I would start with doing some limited proteolysis with a few different
proteases followed by Mass Spec analysis of the fragments to identify
loops that are readily cut.
There is lots of info and published protocols, see e.g.
http://biowww.net/protocols/proteomics/mass_spectrometry/protein_digestion/
Flip
Op 7/7/2011 00:51, Obayed Ullah schreef:
Hi all
I am thinking to delete flexible loop for crystallization of my
protein. But i am not sure how to decide which area i should delete.
This protein have around 20% sequence identity with other solved
structure. Can anybody suggest me how to proceed for that. Is there
any established strategy for that one?
Obayed Ullah
