Better yet, RbBr? and pH MES, MOPS, and HEPES with RbOH?
I haven't checked the price.
Jacob Keller wrote:
Is there any reason why crystallographers have not routinely
substituted NaBr for NaCl in protein crystallization stocks, or even
pH'd their TRIS with HBr, if there is no NaCl? Wouldn't it make a lot
of sense, since there would always be a possibility for a Br-
derivative, and the price difference is pretty small? Also, a data set
at the Br- peak would always be able to distinguish "heavy H2O" from
weak halide sites once the structure was solved, even if the sites
were not good enough for phase determination themselves.
JPK
On Wed, Jun 15, 2011 at 7:35 AM, Stephen Graham<sc...@cam.ac.uk> wrote:
You might also want to try:
http://www.ncbi.nlm.nih.gov/pubmed?term=12499536
Cheers,
Stephen
On 15 June 2011 02:09, Robbie Joosten<robbie_joos...@hotmail.com> wrote:
Hi Wolfram,
This was an early study on the subject:
http://www.ncbi.nlm.nih.gov/pubmed/8594192
The software is still accessible via the STAN server.
Cheers,
Robbie
Date: Tue, 14 Jun 2011 17:51:21 -0400
From: wtem...@gmail.com
Subject: [ccp4bb] non-waters among structured solvent atoms
To: CCP4BB@JISCMAIL.AC.UK
Dear colleagues,
following a discussion in our lab, I have volunteered to dig out
articles from the literature about erroneous assignments of non-water
entities such as metal ions, halides in protein models. For example I
have the faint recollection that data mining of the PDB for suspect
"water" assemblies matching the geometry of coordinated cations has
previously been described. But none of my google searches has turned
up the references I was looking for. Could someone point me in the
right direction, please?
Many thanks,
Wolfram Tempel
--
Dr Stephen Graham
1851 Research Fellow
Cambridge Institute for Medical Research
Wellcome Trust/MRC Building
Addenbrooke's Hospital, Hills Road
Cambridge, CB2 0XY, UK
Phone: +44 1223 762 638
--
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
cel: 773.608.9185
email: j-kell...@northwestern.edu
*******************************************
