Hi Careina 30% of the origin peak is fairly respectable. You could probably make it higher by varying the high res cutoff. You have 2 Ang: it may be that the NCS doesn't extend to that resolution, so making it a bit lower might improve the signal/noise ratio. It would be nice to see the plot of the kappa=180 section, but from the log file it's clear you have a lot of NCS 2-folds in the ab plane (kappa=180, omega=90), so my guess would be a 222 tetramer aligned with an NCS 2-fold parallel to the c axis. If so this should give you an translational NCS peak somewhere in the z=1/2 Harker section of the native Patterson. From the solvent content how many molecules in the a.u. do you expect?
Cheers -- Ian On Fri, Jun 3, 2011 at 2:01 PM, Careina Edgooms <careinaedgo...@yahoo.com>wrote: > Dear ccp4 members > > I have question about how to interpret polarrfn log. I wish to know if my > crystal display NCS. I am not sure how to interpret the file. I see it have > two peak, one is origin and the other is not that high to me. I have attach > copy of the file. If someone could assist me to understand the file it would > be appreciated. > > best > Careina >
