1) you can calculate the approximate unit cell parameter(s) from the spots. I am guessing they will come out at 6-12 A (hard to tell from your image w/o actual distance, wavelength etc). 2) this seems to be a diffractogram of a crystal stack where about a dozen or two of thin plates are stuck together with a relatively good alignment in two dimensions and a variable alignment in the third 3) this seems to be a synchrotron image (based on the detector tiling) but the spots are not all Supernova-bright, which pretty much rules out ammonium sulfate crystals (and also the d-spacing is to large for simple salt).
So I'd guess you're shooting crystals of a small organic molecule, like a detergent would be (but could be something else too). Artem On Mon, May 30, 2011 at 4:21 AM, Bingfa Sun <[email protected]> wrote: > Dear All, > > > > I have got crystals in a condition with about 1.7 M Ammonium Sulfate, and > my buffer contains Hepes, NaCl and glycerol. Initial crystals are extremely > thin, needlelike while adding of some detergent resulted in thicker, rodlike > crystals. However, diffraction pattern is quite weird which do not seem to > be a protein crystal or a typical salt crystal. Is this a detergent crystal > or an ammonium sulfate crystal? > > > > Diffraction images are in the attachment. > > > > Thank you all in advance. > > > > Bingfa > > >
