Hi,
This article says it is the Human Rhinovirus HRV3C Protease:
https://wasatch.biochem.utah.edu/chris/links/PrescissionProteaseProtocol.doc
The genome of this virus is here:
http://www.ncbi.nlm.nih.gov/nuccore/156254956
The gene record for the polyprotein is here:
http://www.ncbi.nlm.nih.gov/gene/5758809
The poly protein sequence is here:
http://www.ncbi.nlm.nih.gov/protein/156254957
Then the 3C protein, ie, the protease is this guy:
http://www.ncbi.nlm.nih.gov/protein/156254957?from=1502&to=1684&report=gpwithparts
If you blast this sequence in PDB, you will get 43 hits. I suggest you to
have a look at some of the structures to decide how to design your
construct. This protease shares some characteristics with the TEV protease,
for example, Cys protease, having no disulfide bonds but multiple Cys
residues, recognizing a fairly hydrophobic sequence. So I guess you may also
want to consult the people who were successful with designing and producing
TEV protease constructs. My experience with TEV is: 1) a self-cleavable MBP
fusion (See TEV FAQ, by Dr. Waugh) works very well, although a non-cleavable
MBP fusion also works; 2) do not send the protein to periplasm.
Zhijie
--------------------------------------------------
From: "Lieh Yoon Low" <liehy...@gmail.com>
Sent: Wednesday, May 25, 2011 4:36 PM
To: <CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] PreScission Protease protein sequence
Dear All,
I apologize for a non-crystallography question, but does anyone know the
sequence of the PreScission Protease? I would like to make it for use in
my own lab. It is just too expensive to purchase from GE!
Thanks
ray
