Dear CCP4 community, I have a question a bit off CCP4 topic, but that could use so expert input. While discussing about a bacterial secreted hemophore (heme scavenging protein) for which the apo form has been solved, it seems that attempts to obtain the Heme bound form are failing; in fact during data collection on a supposed Heme bound form, not only the Iron state changes (reduction), but the Heme position seems to be affected too. Would anybody have some experience with such a system and care to send some inputs (off line is fine as we are here a bit off topic)? Thank you in advance for your help. Best regards and Happy Building, Steph -- Stephane B. Richard, Ph.D., VP Business Development MEDIT US, 7985 Dunbrook Rd., Ste A, San Diego, CA 92126, USA MEDIT SA, 2 rue du Belvedere, 91120 Palaiseau, France http://www.linkedin.com/in/stephanebrichard Web: http://www.medit-pharma.com/ Email: [email protected] Cell: +1(858)342.6807
________________________________ From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Nian Huang Sent: Monday, April 11, 2011 2:20 PM To: [email protected] Subject: Re: [ccp4bb] immobilized DNA resin Heparin simulates the structure of DNA and RNA, so it has nonspecific affinity towards DNA or RNA binding protein. It has also been used as DNAse or RNase inhibitor but it is not very good one. Nian Huang, Ph.D. UT Southwestern Medical Center On Sat, Apr 9, 2011 at 7:44 PM, Alexandra Deaconescu <[email protected]> wrote: Hello ccp4 enthusiasts: I am afraid this is a non-ccp4 related question. Can anyone recommend an immobilized dsDNA chromatographic resin for purification of DNA-binding proteins? GE seems to have something - I was wondering if people have other recommendations? In the age of GST and His tags etc., these are not very much used, but I do not have a tag in this case... Thanks a lot, Alex
