PS, it might be a good time to start an additive screen.
--------------------------------------------------
From: "Jun Yong Ha" <j...@princeton.edu>
Sent: Tuesday, April 12, 2011 7:56 AM
To: <CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] Reproducing crystals.
Hi all,
Recently, I produced crystals with MBClass1-64 which contains PEG4000,
HEPES-Na and NaCl. But, I struggled to reproduce crystals. I tried to set
up tray with different batch of solution. I got the crystals only from
2008 solution, but not from fresh ones. I asked technical service of
Qiagen, but they did not have any stock.
pH between fresh and old solution is the same. I could reproduce crystals
with this old solution 100% when setting up.
Do you have any experience like this? Is PEG4000 degraded or oxidized?
Please help me.
Thanks in advance.
