Try fitting a xylitol molecule in it but watch out for the distortions caused by proximity to symmetry axis.
Artem On Thu, Mar 31, 2011 at 1:16 PM, Shu XU <xushuh...@gmail.com> wrote: > Hi, there. > I'm refining a 1.76 A structure. The r-work stuck at 21%, rfree is 24% > after adding waters. > But there is something between the interfaces of the dimers. > The space group is I4122, so it looks like something small, five or > six atoms all together (without hydrogens). > I tried to fit Tris in, however, it didn't look good at all. > The crystal came out from Tris-cl buffer, ammonium sulfate, and there > is some NaCl in the protein buffer too. > The crystals were dropped into 20% xylitol and flash frozen. > Fo-Fc maps are attached at 3 sigma level. > Let me know what you're thoughts are. > Thank you for your help. > > Shu > > > -- > Shu Xu > Ph.D candidate in Chemistry > Department of Chemistry > The University of Toledo > Toledo, OH 43606 > Tel 419-530-1524 >
