Just an offshoot of the same Question.. I would like to ask whether the same applies for GST-tag digestion using thrombin.. No agitation gives better results in the above case too... Any personal experiences....
On Thu, Mar 31, 2011 at 11:29 AM, Klaus Piontek < klaus.pion...@ocbc.uni-freiburg.de> wrote: > And not at full moon! > > Klaus > > > Am 31.03.2011 um 16:23 schrieb Xiaopeng Hu: > > Our experience is do not shake the tube during TEV cleavage,I dont know > why, but it does help. > > xiaopeng > > > Dr. Klaus Piontek > Albert-Ludwigs-University Freiburg > Institute of Organic Chemistry and Biochemistry, Room 401 H Albertstrasse > 21 > D-79104 Freiburg Germany > Phone: ++49-761-203-6036 > Fax: ++49-761-203-8714 > Email: klaus.pion...@ocbc.uni-freiburg.de > Web: http://www.chemie.uni-freiburg.de/orgbio/w3platt/ > >
