Hi Guys, we are learning to work with Sf9 cells and Carol in my lab wanted me to ask you the following question. Many thanks for any help, G
I need to titer a baculovirus stock in my suspension-adapted Sf9 cells. I know that these can be encouraged to attach better to tissue culture plastic if they have added FBS (about 10%), but am not sure that they will not be migrating and hiding plaques. Does anyone have suggestions about how to keep them more firmly anchored during the baculovirus titration, or about another cell line that we could use instead?
