Dear Gauri This may be the saturation point of your protein and beyond this it will start precipitating in a particular buffer. Try to set the crystallization trial (if it is your application ) and select those condition in which you see clear drop. Change the purification buffer with that condition buffer. It may work .. Else you can use Sucrose (0.5-2M), PEG6000 (5-10%), glycerol (5-10%) etc in different combination. all d best
Vikrant ----------------------------------------------------------------------------------- Senior Research Fellow (CSIR) Varma Lab Cancer Research Institute Advance Centre for Treatment, Research and Eduction in Cancer (ACTREC) Tata Memorial Hospital, Kharghar, Navi Mumbai-410210 ################################################ ________________________________ From: gauri misra <kamga...@gmail.com> To: CCP4BB@JISCMAIL.AC.UK Sent: Wed, 23 March, 2011 11:11:55 PM Subject: [ccp4bb] protein aggregation Hi, What are the different methods to prevent protein aggregation while concentrating so as to increase the concentration of the protein? I have some idea of adding EDTA and charged amino acids like L-Arg and L-Glu. I would appreciate if the readers share their experiences. Thanks! Gauri
