Dear All, I apologize if the questions has already been asked on this forum. We are purifying a membrane that seems prone to proteolysis. Although we use Protease Inhibitor cocktails during lysis and the first step of purification we get rid of them after and only keep PMSF and EDTA as general anti-protease control agents. I am considering reincluding the cocktail of inhibitors at the last purification step (a size exclusion in our case) and was wondering if having this infamous mixture of peptidic inhibitors (for the most part) around during crystallization would be a problem: specifically getting crystals of these inhibitors. Does anyone have extended experience in this matter.
Many thanks in advance. -- Pascal F. Egea, PhD Assistant Professor UCLA, David Geffen School of Medicine Department of Biological Chemistry 314 Biomedical Sciences Research Building office (310)-983-3515 lab (310)-983-3516 email pe...@mednet.ucla.edu
