Hi Sadaf It is also possible that the features you observe are also due to anisotropy of the ADPs. At 1.3A resolution you can perform anisotropic refinement. Give it a go and se if it helps.
Best Roberto On 6 Mar 2011, at 18:57, sadaf iqbal wrote: Hello everyone, Recently, i have solved a protein structure at 1.3 Å resolution. I have encountered one problem in this structure while refining in COOT. There are three disulfide bridges in my structure and if i used 2.0 Å resolution range in refmac then the density around disulfide bridges is ok but if i used 1.3 Å resolution range in refmac then red density is appeared along with blue one. Can anybody help me in this regard? I already played by increasing temperature factor of sulfur atoms but red density is still there. Thanks in advance Sadaf Iqbal PhD Scholar ICCBS, University of Karachi, Pakistan. & Visiting Scientist University of Hamburg, Germany.
