Hi,
There's a whole bunch of programs that can help you "out there".
The 2 methods I think of right now are DISPROT (there's a server I
believe, http://www.ist.temple.edu/disprot/ ) - Must admit I haven't
been to that one for quite a while; DISPROT provides areas of your
sequence with high probability of "disorder"
hydrophobic cluster analysis.
There are many others as well, can't think of them right now.
Also, common sense (like trying to crystallise with and without tags)
can be helpful.
You may have to try to crystallise several constructs. And there's more
than just "compact and stable" to crystallisation. Monodispersity is
quite important too.
Fred.
Xianhui Wu wrote:
Dear all,
Before we try to study the crystal structure of an unknown protein,
we need to determine the sequence that can fold into a compact and
stable 3D domain. What kinds of methods can we choose?
--
Best regards,
XH Wu
