Dear all, I have a question concerning removal of a his-tag sequence. We have crystallized a protein with an important feature at the C-terminal part of the protein. Unfortunately, we cannot express it with a N-terminal his-tag, only with a C-terminal his-tag.
Therefore we are looking for a protease which cleaves off the sequence without leaving any extra amino acid on the C-terminus of our protein. Meaning we obtain really the wild type protein. Does anyone know about a protease or cleavage site which is completely removed? Many thanks in advance Phil
