Dear Simon,
If I'm right, the first step in the solution of the 50S particle by Nenad Ban was MR with EM envelope. There are also a few articles in Acta Cryst (roughly last 15 years) on this subject. Best regards, Sacha Urzhumtsev -----Message d'origine----- De : CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] De la part de Simon Kolstoe Envoyé : jeudi 20 janvier 2011 14:36 À : CCP4BB@JISCMAIL.AC.UK Objet : [ccp4bb] Low resolution MR Dear CCP4bb, I've just started on a new project and was rather excited to see protein spots for my first few crystals at Diamond the other day. The only problem is that the reflections only went out to 8.5A. As it should be possible to get a solution using molecular replacement (and whilst I am waiting for new and hopefully better crystals to grow) will MR work with only 8.5A data (100% complete)? I've spent the last couple of days playing with phaser and molrep but not had much luck so far, so was wondering if 8.5A might be a lost cause for MR? Thanks, Simon
