Let's say I need to insert two G between resi 98 and 99. I tried in the way as Herman suggest: break the chain in the domain boundary, add terminal residues at the N-terminus of the 2nd domain. But then there is no space to accommodate the inserted residues, the two glycerin backbones overlap with resi 97 and 98. Is there a way to shift the whole 1st domain a bit ?
On Thu, Jan 13, 2011 at 12:16 PM, <herman.schreu...@sanofi-aventis.com>wrote: > use coot: calculate - model/fit/refine - add terminal residue. > this will add alanines. you can change these to glycines with the simple > mutate option in the same pop up menu. > > best, > HErman > > ------------------------------ > *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of *Qing > Chen > *Sent:* Thursday, January 13, 2011 11:58 AM > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [ccp4bb] creat a model with insertion > > Dear all, > > I have the wt protein structure that contains two domains. I want to creat > a model with two glycerin inserted between the two domains. > Which software or webserver could do this? > Pls help. > > > Qing Chen > >
