Dear Bernhard,
I must say that I find the "super-resolution" claims in this paper a
bit of a conjuring trick. If the final refined model has greater accuracy
than one would expect from the resolution of the data it has been refined
against, it is because that extra accuracy has been lifted from the higher
resolution data that were used to refine the structure on the basis of which
the elastic network restraints were created.
Should we then say that we achieve super-resolution whenever we refine
a macromolecular structure using Engh & Huber restraints, because these
enable us to achieve distance accuracies comparable with those in the small
molecules structures in the Cambridge Structural Database?
Perhaps I have missed an essential point of this paper.
With best wishes,
Gerard.
--
On Thu, Dec 23, 2010 at 12:25:26PM -0800, Bernhard Rupp (Hofkristallrat a.D.)
wrote:
> Oops I am outdated: Axel just emailed me that he describes an improved
> coordinate estimate beyond the Rayleigh criterion in his recent paper
>
> Schroder GF, Levitt M, & Brunger AT (2010) Super-resolution biomolecular
> crystallography with low-resolution data. Nature 464(7292), 1218-1222.
>
> For the deformable elastic network (DEN) refinement, see his ref 14
>
> Schroder GF, Brunger AT, & Levitt M (2007) Combining efficient
> conformational sampling with a deformable elastic network model facilitates
> structure refinement at low resolution. Structure 15(12), 1630-1641.
>
> BR
--
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