Hi Liu Looks like (on the images you show) that you have a nice conformational difference for some of the helices between the 2 structures...
Happy Hols Gina -----Original Message----- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Justin Hall Sent: Monday, December 20, 2010 8:57 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] If it is a new structure? Hi Liu, If I understand your question correctly, youre asking "how different do two structures need to be for one to be "new'". If by "new" you mean a new fold, then the answer is NO. Your structure and the homolog have the same fold. However, if your structure is the first structure of a protein in a new class, then your structure is a new insight for that reason (e.g. it is the first structure of a Unobtainium-metalloprotease). If it is not the first structure of a protein from a new class, lets say a previous structure of Unobtainium-metalloprotease has been solved using H. sapiens' sequence, but your protein is the first D. melanogaster ortholog solved, then your structure is a new insight for that reason. So, in a nut-shell, I guess what I am saying is that your protein is not a new fold, but is almost certainly "new" by some qualification, and you will know best what that qualification is. I hope that helps, cheers and happy holidays~ ~Justin > On 20/12/2010 10:49, <Liu Zhao > wrote: >> The structure of my protein is as shown as the purple one. Another one >> ,as shown as green,is homologous .But the structure of my protein can't >> be obtained by using molecular replacement. And both structures have >> much different, especially in B chain. If my structure is a new one? >> thank you for help. Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
