Hi Afshan, you didn't mention the resolution, which is essential. For ultra-high resolution structures the average B may be well less than 10, and <B> less than ~4-5 is the condition to see the bonding density (see aldose reductase, 1uso, for instance). While a structure at 3.5A resolution may have B-factors larger than 100 or so.
Pavel. On Wed, Dec 15, 2010 at 10:24 AM, Afshan Fayazi <afshan...@yahoo.com> wrote: > > > **Actually i have sioved my structure of the protein but the B factor > (temperature) overall is very high . it sis more than 50 so how can i > reduce this or what is the acceptable B factor for the submission > > > Best Regards > > AFSHAN > > > <http://de.mc517.mail.yahoo.com/mc/compose?to=bet...@unisgi1.desy.de> > > >
