Re: [ccp4bb] protein concentration in crystal

[Roger Rowlett]

Sounds like a good units conversion problem for Introductory Chemistry. Basically, you can get molecules per cubic angstrom from your unit cell analysis (molecules per unit cell and cubic angstroms per unit cell) and convert to the units of your choice, mg/cm^3, mol/L (actually mol/dm^3 in this case), or whatever. Or you can use your Matthews number and start from A^3/Dalton. Cheers. On 12/13/2010 3:07 PM, Teresa De la Mora wrote: Hi all I'm looking for a way to calculate the protein concentration in a single crystal. So what I'm thinking is to use the Matthew's number to calculate how many molecules inside the crystal, then multiply that number by Avogadro's number to get moles then divide by volume of crystal. Is this approach correct? Thank you for your advice/suggestion Happy holidays! :) Teresa Teresa De la Mora-Rey Ph.D. Dept. Medicinal Chemistry  University of Minnesota  8-101 Weaver-Densford Hall  308 Harvard St. SE, Minneapolis, MN 55455  Lab phone (612) 626-5226  "If you never did you should. These things are fun and fun is good" Dr. Seuss -- Roger S. Rowlett Professor Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: rrowl...@colgate.edu