Dear,I was wondering if anybody has experienced before the leakage of Ni- ions (from a Ni-NTA column) and additionally binding to specific sites in the protein structure..?
Many thanks Regards Kristof -------------------------------------- Kristof Van Hecke, PhD Biomoleculaire Architectuur Celestijnenlaan 200 F B-3001 Heverlee (Leuven) Tel: +32(0)16327477 --------------------------------------